Caracterização farmacológica da desnervação e reinervação simpática do ducto deferente de rato

Abstract

The vas deferens of rat (DDR) has rich sympathetic innervation, being the main ATP noradrenaline and mediators involved in neurotransmission in the organ. Several studies have shown that in the DDR surgically denervated changes occur in tissue contraction. However, there are scarce studies that characterized these changes and followed for a longer time to consider the period of reinnervation of the fabric. This study set out to do in DDR surgically denervated, a temporal analysis of the changes in contractile ability of starting the study 4 days after surgery and reinnervation process for 84 days after the procedure, in order to establish new parameters indicative of reinnervation of the organ. For this the DDR will be denervated according to the technique described by Kasuya et al. (1969), the animals were anesthetized with ketamine and Xylazina, then an incision was made in the region above pubis, exposing the seminal vesicle and the DDR. With the help of tweezers, the nerve and artery that travels along the vas deferens of rat were separated from the organ for about 1 cm, a small amount of phenol 5% was applied in this region, and the abdominal wall was sutured. Non-operated animals were used as controls. After the procedure the mice were housed in cage and taken to the biologist to be used in experiments. In different days after the procedures (4, 7, 14, 21, 28, 56 and 84) the animals were euthanized in CO2 Chamber the DDRs removed, cleaned and assembled in isolated organ equipment where they were subjected to the action of KCl and high frequency electric stimulus. We observed that there was no change in the areas of the contraction curves of the denervated animals in comparison with those of the control group. . The data indicate that the contractility of the organ is not affected by the surgical denervation of the sympathetic component of the organ, which leads us to suggest that there should be a compensation of the contractile machinery in order to maintain the functionality of the organ, even under conditions of sympathetic neurotransmission failure.