Insights into Antimicrobial Peptides from Limacus flavus Mucus


External affiliation
Publication type
Article
Language
English
Access rights
Restricted Access
Appears in Collections:
Metrics
Abstract
Antimicrobial peptides have been a major research subject since the rise of antimicrobial resistance as a major public health problem. These molecules are considered a potential therapeutic source of antibiotics with broad-spectrum activity against microorganisms. Two antimicrobial peptides were isolated from the mucus of the Limacus flavus slug. The mucus was obtained by thermal shock, lyophilized and extracted with acetic acid. The supernatant was prefractionated in Sep-Pak and shortly thereafter fractionated by reverse-phase high-performance liquid chromatography. The manually obtained fractions were used in antimicrobial and cytotoxic assays and finally subjected to mass spectrometry (MS/MS). Characterization was performed by bioinformatics analysis with the tool Peaks®X + and by comparison with the NCBI and UniProt-SwissProt databases. Additionally, the physicochemical parameters of the samples were evaluated with online programs. Two fractions comtained antimicrobial peptides with the ability to inhibit Micrococcus luteus A270; both samples, LFMP-001 and LFMP-002, were hydrophilic molecules consisting of fewer than 20 residues. Comparison of the SDS-PAGE and Peaks®X + data showed that both had Mw < 3 kDa. In summary, this study presents data on the isolation and characterization of antimicrobial peptides from a slug and shows their potential against gram-positive bacteria.
Reference
Hayashida PY, Silva Junior PI. Insights into Antimicrobial Peptides from Limacus flavus Mucus. Curr. Microbiol.. 2021 June;in press. doi:10.1007/s00284-021-02552-3.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/3828
Journal title
Issue Date
2021


Files in This Item:

Existing users please Login
10.1007s00284-021-02552-3.pdf
Description:
Size: 2.52 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show full item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.