Genetic and phenotypic characterization of an enteroaggregative Escherichia coli isolated from a urinary tract infection
Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor | Lab. Bacteriologia | pt_BR |
| dc.date.accessioned | 2021-07-22T23:58:45Z | - |
| dc.date.available | 2021-07-22T23:58:45Z | - |
| dc.date.issued | 2021 | pt_BR |
| dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/3890 | - |
| dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
| dc.description.sponsorship | (CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | pt_BR |
| dc.format | pdf, txt, xlsx | pt_BR |
| dc.format.extent | 1.51 MB | pt_BR |
| dc.language.iso | Portuguese | pt_BR |
| dc.language.iso | English | pt_BR |
| dc.publisher | Instituto Butantan | pt_BR |
| dc.rights | Open access | pt_BR |
| dc.title | Genetic and phenotypic characterization of an enteroaggregative Escherichia coli isolated from a urinary tract infection | pt_BR |
| dc.type | Dataset | pt_BR |
| dc.relation.publication | https://repositorio.butantan.gov.br/handle/butantan/4358 | pt_BR |
| dc.contributor.external | (UNIFESP) Universidade Federal de São Paulo | pt_BR |
| dc.contributor.external | (UNESP) Universidade Estadual Paulista Júlio de Mesquita Filho | pt_BR |
| dc.contributor.external | University of Münster | pt_BR |
| dc.identifier.bvscc | BR78.1 | pt_BR |
| dc.identifier.bvsdb | IBProd | pt_BR |
| dc.subject.researchline | Patogenicidade bacteriana | pt_BR |
| dc.contributor.researcher | Elias, Waldir Pereira | pt_BR |
| dc.date.modified | 2021-07-22 | - |
| dc.description.dbindexed | Yes | pt_BR |
| dc.description.file | Biofilm Urine polystyrene (.pdf): absorbance values of biofilm formation in human pooled urine on polystyrene surface. Biofilm Urine glass (.pdf): absorbance values of biofilm formation in human pooled urine on glass surface. Growth curve LB medium (.pdf): growth curve of UPEC-46 and derivative constructions in LB medium – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve DMEM (.pdf): growth curve of UPEC-46 and derivative constructions in DMEM – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve RPMI (.pdf): growth curve of UPEC-46 and derivative constructions in RPMI – absorbance values at 600 nm measured every 30 minutes during 6 hours. Motility (.pdf): halo diameter (cm) of UPEC-46 and derivative constructions on LB agar containing 0.3% agar. Quantitative_adherence HeLa cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HeLa cells – CFU/ml counting. Quantitative_adherence HT-29 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HT-29 cells – CFU/ml counting. Quantitative_adherence 5637 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in 5637 cells – CFU/ml counting. Steps of afpA mutagenesis (.pdf): steps employed to construct the afpA mutant strain using wild-type UPEC-46 – primers and PCR amplicons analyzed by agarose gel electrophoresis. Steps of afpA mutant complementation (.pdf): steps employed to complement the UPEC-46 mutant in afpA strain – PCR amplicons analyzed by agarose gel electrophoresis. Sanger sequencing of afpA mutant-forward (.txt): forward nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant-reverse (.txt): reverse nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant complementation-forward (.txt): forward nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Sanger sequencing of afpA mutant complementation- reverse (.txt): reverse nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Prokka UPEC-46 general data (.txt): general data of whole genome sequence of UPEC-46 using Prokka software. Quast UPEC-46 genome quality (.pdf): quality parameters of UPEC-46 genome sequence using Quast software. UPEC-46 virulence genes hits (.xlsx): analysis of the presence of several E. coli virulence genes in the genome of UPEC-46 – identity and coverage. afpA positive strains final binary matrix (.xlsx): binary matrix of presence/absence of several E. coli virulence genes in the genome of UPEC-46, where zero=absence and 1=presence. RAxML_Phylogenetic Tree E. coli reference strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and reference E. coli strains. RAxML_Phylogenetic Tree UPEC, EAEC and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and UPEC and EAEC reference strains. RAxML_Phylogenetic Tree ST10 and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and ST10 E. coli strains. RAxML_Phylogenetic Tree afpA-positive E. coli strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and afpA-positive E. coli strains. | pt_BR |
| dc.sponsorship.fapesp | 2018/06610-9; 2018/04144-0 | pt_BR |
| item.fulltext | Com Texto completo | - |
| item.grantfulltext | open | - |
| item.languageiso639-1 | Portuguese | - |
| item.languageiso639-1 | English | - |
| item.openairetype | Dataset | - |
| Appears in Collections: | Dados de Pesquisa (Dataset) | |
Files in This Item:
afp positive strains final binary matrix.xlsx
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RAxML_Phylogenetic Tree afp-positive E. coli strains and UPEC-46.txt
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RAxML_Phylogenetic Tree E. coli reference strains and UPEC-46.txt
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Sanger sequencing of afpA mutant complementation - foward.txt
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Sanger sequencing of afpA mutant complementation - reverse.txt
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