Genetic and phenotypic characterization of an enteroaggregative Escherichia coli isolated from a urinary tract infection

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dc.contributorLab. Bacteriologiapt_BR
dc.date.accessioned2021-07-22T23:58:45Z-
dc.date.available2021-07-22T23:58:45Z-
dc.date.issued2021pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3890-
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.formatpdf, txt, xlsxpt_BR
dc.format.extent1.51 MBpt_BR
dc.language.isoPortuguesept_BR
dc.language.isoEnglishpt_BR
dc.publisherInstituto Butantanpt_BR
dc.rightsOpen accesspt_BR
dc.titleGenetic and phenotypic characterization of an enteroaggregative Escherichia coli isolated from a urinary tract infectionpt_BR
dc.typeDatasetpt_BR
dc.relation.publicationhttps://repositorio.butantan.gov.br/handle/butantan/4358pt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.externalUniversity of Münsterpt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlinePatogenicidade bacterianapt_BR
dc.contributor.researcherElias, Waldir Pereirapt_BR
dc.date.modified2021-07-22-
dc.description.dbindexedYespt_BR
dc.description.fileBiofilm Urine polystyrene (.pdf): absorbance values of biofilm formation in human pooled urine on polystyrene surface. Biofilm Urine glass (.pdf): absorbance values of biofilm formation in human pooled urine on glass surface. Growth curve LB medium (.pdf): growth curve of UPEC-46 and derivative constructions in LB medium – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve DMEM (.pdf): growth curve of UPEC-46 and derivative constructions in DMEM – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve RPMI (.pdf): growth curve of UPEC-46 and derivative constructions in RPMI – absorbance values at 600 nm measured every 30 minutes during 6 hours. Motility (.pdf): halo diameter (cm) of UPEC-46 and derivative constructions on LB agar containing 0.3% agar. Quantitative_adherence HeLa cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HeLa cells – CFU/ml counting. Quantitative_adherence HT-29 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HT-29 cells – CFU/ml counting. Quantitative_adherence 5637 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in 5637 cells – CFU/ml counting. Steps of afpA mutagenesis (.pdf): steps employed to construct the afpA mutant strain using wild-type UPEC-46 – primers and PCR amplicons analyzed by agarose gel electrophoresis. Steps of afpA mutant complementation (.pdf): steps employed to complement the UPEC-46 mutant in afpA strain – PCR amplicons analyzed by agarose gel electrophoresis. Sanger sequencing of afpA mutant-forward (.txt): forward nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant-reverse (.txt): reverse nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant complementation-forward (.txt): forward nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Sanger sequencing of afpA mutant complementation- reverse (.txt): reverse nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Prokka UPEC-46 general data (.txt): general data of whole genome sequence of UPEC-46 using Prokka software. Quast UPEC-46 genome quality (.pdf): quality parameters of UPEC-46 genome sequence using Quast software. UPEC-46 virulence genes hits (.xlsx): analysis of the presence of several E. coli virulence genes in the genome of UPEC-46 – identity and coverage. afpA positive strains final binary matrix (.xlsx): binary matrix of presence/absence of several E. coli virulence genes in the genome of UPEC-46, where zero=absence and 1=presence. RAxML_Phylogenetic Tree E. coli reference strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and reference E. coli strains. RAxML_Phylogenetic Tree UPEC, EAEC and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and UPEC and EAEC reference strains. RAxML_Phylogenetic Tree ST10 and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and ST10 E. coli strains. RAxML_Phylogenetic Tree afpA-positive E. coli strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and afpA-positive E. coli strains.pt_BR
dc.sponsorship.fapesp2018/06610-9; 2018/04144-0pt_BR
item.grantfulltextopen-
item.languageiso639-1Portuguese-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeDataset-
Appears in Collections:Dados de pesquisa


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Biofilm DMEM polystyrene.pdf
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Biofilm DMEM glass.pdf
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Biofilm Preconditioned glass.pdf
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Biofilm Preconditioned polystyrene.pdf
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Biofilm Urine glass.pdf
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Biofilm Urine polystyrene.pdf
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Growth curve DMEM.pdf
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Growth curve LB medium.pdf
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Growth curve RPMI.pdf
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Kinetic biofilm DMEM 1% α-D-man.pdf
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Kinetic biofilm DMEM Absence.pdf
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Kinetic biofilm Urine 1% α-D-man.pdf
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Kinetic biofilm Urine Absence.pdf
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Motility.pdf
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Prokka UPEC-46 general data.txt
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Quantitative_adherence 5637 cells.pdf
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Quantitative_adherence HeLa cells.pdf
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Quantitative_adherence HT-29 cells.pdf
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Quast UPEC-46 genome quality.pdf
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Steps of afpA mutant complementation.pdf
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Steps of afpA mutagenesis.pdf
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afp positive strains final binary matrix.xlsx
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RAxML_Phylogenetic Tree ST10 and UPEC-46.txt
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Sanger sequencing of afpA mutant - foward.txt
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UPEC46 virulence genes hits.xlsx
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