Role of the serine protease Sat in the pathogenesis of sepsis caused by Escherichia coli

Role of the serine protease Sat in the pathogenesis of sepsis caused by Escherichia coli

Full metadata record

DC Field	Value	Language
dc.contributor	Lab. Bacteriologia	pt_BR
dc.date.accessioned	2021-10-01T20:28:06Z	-
dc.date.available	2021-10-01T20:28:06Z	-
dc.date.issued	2021	pt_BR
dc.identifier.uri	https://repositorio.butantan.gov.br/handle/butantan/3947	-
dc.description.sponsorship	(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico	pt_BR
dc.description.sponsorship	(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo	pt_BR
dc.format	pdf	pt_BR
dc.language.iso	English	pt_BR
dc.rights	Open access	pt_BR
dc.title	Role of the serine protease Sat in the pathogenesis of sepsis caused by Escherichia coli	pt_BR
dc.title.alternative	Papel da serinoprotease Sat na patogênese da sepse causada por Escherichia coli	pt_BR
dc.type	Dataset	pt_BR
dc.relation.publication	https://repositorio.butantan.gov.br/handle/butantan/4264	pt_BR
dc.contributor.external	(UNIFESP) Universidade Federal de São Paulo	pt_BR
dc.contributor.external	University of Birmingham	pt_BR
dc.subject.keyword	Escherichia coli	pt_BR
dc.subject.keyword	Bacteremia	pt_BR
dc.subject.keyword	sepsis	pt_BR
dc.subject.keyword	Sat	pt_BR
dc.subject.keyword	SPATE	pt_BR
dc.sponsorship.butantan	CNPq 142053/2015-5	pt_BR
dc.identifier.bvscc	BR78.1	pt_BR
dc.identifier.bvsdb	IBProd	pt_BR
dc.subject.researchline	Patogenicidade bacteriana	pt_BR
dc.contributor.researcher	Elias, Waldir Pereira	pt_BR
dc.description.dbindexed	Yes	pt_BR
dc.description.file	EC071 25% serum resistance – CFU.mL-1 counting (.pdf): colony forming units counting for bacterial survival after serum resistance assay. EC071 25% serum resistance – graph (.pdf): graphic representation of colony forming units counting for bacterial survival after serum resistance assay. EC071 plasmid analysis (.pdf): plasmid extraction analysis in an agarose gel. Genetic constructions and modifications – agarose gels: PCR, digestion and plasmid extraction products analysis in agarose gels. Growth curve – CFT073 (.pdf): growth curve of CFT073 in LB medium. Growth curve – EC071 and derivative strains (.pdf): growth curve of EC071 and derivative strains in LB medium. Growth curve – MG1655 and derivative strains (.pdf): growth curve of MG1655 and derivative strains in LB medium. Growth curve graphs – CFT073, MG1655 and derivative strains (.pdf): growth curve of CFT073, MG1655 and derivative strains in LB medium graphic representation. Growth curve graphs – EC071 and derivative strains (.pdf): growth curve of EC071 and derivative strains in LB medium graphic representation. Murine sepsis model – survival graph (.pdf): graphic representation of animal survival in a EC071 and derivative strains induced sepsis model. Murine sepsis model – survival statistics (.pdf): statistical analysis of animal survival in a EC071 and derivative strains induced sepsis model. pCF2 insertion in EC071 sat confirmation (.pdf): alignment of sequenced PCR products for pCF2 interruption of the sat gene in EC071 chromosome. pCF4 site-directed mutagenesis confirmation (.pdf): alignment of sequenced PCR products for nucleotide alterations in pCF4 sat gene. Proteolytic assays Immunoblotting (.pdf): detection of cleavage products after Sat and complement targets incubation. Sat or Sat’ production by pCF3 and pCF4 harboring strains (.pdf): detection of Sat ou Sat’ in TCA-treated culture supernatants of pCF3 and pCF4 harboring strains by SDS-PAGE, followed by silver nitrate staining and Immunoblotting. Sat production by EC071 and derivative strains (.pdf): detection of Sat in TCA-treated culture supernatants of EC071 and derivative strains by SDS-PAGE, followed by silver nitrate staining and Immunoblotting. Sat purification – mass spectrometry (.pdf): mass spectrometry analysis report for Sat identification in post-purification fractions. Sat purification – Silver staining and Immunoblotting: Sat detection in post-purification fractions by SDS-PAGE, followed by silver nitrate staining and Immunoblotting.	pt_BR
dc.sponsorship.fapesp	2017/14821-7	pt_BR
item.grantfulltext	open	-
item.openairetype	Dataset	-
item.languageiso639-1	English	-
item.fulltext	Com Texto completo	-
Appears in Collections:	Dados de Pesquisa (Dataset)