Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli
(UVA) University of Virginia ; (IMSS) Instituto Mexicano del Seguro Social ; (BUAP) Benemérita Universidad Autónoma de Puebla ; (UF) University of Florida ; (UFMA) Universidade Federal do Maranhão ; (UNESP) Universidade Estadual Paulista Júlio de Mesquita Filho ; (IPN) Instituto Politécnico Nacional
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The interaction of enteroaggregative Escherichia coli (EAEC) strains with the colonic gut mucosa is characterized by the ability of the bacteria to form robust biofilms, to bind mucin, and induce a local inflammatory response. These events are mediated by a repertoire of five different aggregative adherence fimbriae variants (AAF/I-V) typically encoded on virulence plasmids. In this study, we report the production in EAEC strains of a new YehD fimbriae (YDF), which is encoded by the chromosomal gene cluster yehABCD, also present in most E. coli strains. Immuno-labelling of EAEC strain 042 with anti-AAF/II and anti-YDF antibodies demonstrated the presence of both AAF/II and YDF on the bacterial surface. We investigated the role of YDF in cell adherence, biofilm formation, colonization of spinach leaves, and induction of pro-inflammatory cytokines release. To this aim, we constructed yehD deletion mutants in different EAEC backgrounds (strains 17-2, 042, 55989, C1010, 278-1, J7) each harbouring one of the five AAFs. The effect of the YDF mutation was strain dependent and AAF independent as the lack of YDF had a different impact on the phenotypes manifested by the different EAECs tested. Expression of the yehABCD operon in a E. coli K12 ORN172 showed that YDF is important for biofilm formation but not for adherence to HeLa cells. Lastly, screening of pro-inflammatory cytokines in supernatants of Caco-2 cells infected with EAEC strains 042 and J7 and their isogenic ΔyehD mutants showed that these mutants were significantly defective in release of IL-8 and TNF-α. This study contributes to the understanding of the complex and diverse mechanisms of adherence of EAEC strains and identifies a new potential target for preventive measures of gastrointestinal illness caused by EAEC and other E. coli pathogroups.
Soria-Bustos J, Saitz W, Medrano A, Lara-Ochoa C, Bennis Z, Monteiro-Neto V, et al. Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli. Environ. Microbiol. 2021 Aug;in press. doi:10.1111/1462-2920.15737.
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