Quantifying the affinity of trypanosoma cruzi RPA-1 to the single-stranded DNA overhang of the telomere using surface plasmon resonance

Vitarelli, Marcela de Oliveira; Elias, Maria Carolina

Quantifying the affinity of trypanosoma cruzi RPA-1 to the single-stranded DNA overhang of the telomere using surface plasmon resonance

Author

Vitarelli, Marcela de Oliveira; Elias, Maria Carolina

Butantan affiliation

(LCC) Lab. Ciclo Celular ; (CeTICS) Centro de Toxinas, Resposta-imune e Sinalização Celular

Publication type

Book chapter

Language

English

Access rights

Restricted access

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Abstract

Surface plasmon resonance (SPR) biosensors provide real-time binding affinity measurements between a pair of biomolecules, characterizing its interaction dynamics. An example of Trypanosoma cruzi’s RPA-1 and a single-stranded DNA telomere sequence is presented with detailed guidelines and fundamentals for SPR technology.

Link to cite this reference

https://repositorio.butantan.gov.br/handle/butantan/4211

Keywords

Biosensor; Surface plasmon resonance; Protein-DNA interactions; RPA; Single-stranded DNA; Kinetics; Trypanosoma cruzi

Funding agency

(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo ; (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico

Issue Date

2021

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