Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

Publication type
Access rights
Open access
Terms of use
Appears in Collections:
Background Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity. Conclusions The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.
Santos GB, Silva ED, Kitano ES, Battistella ME, Monteiro KM, Lima JC, et al. Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis. Parasit. Vectors. 2022 Mar;15:99. doi:10.1186/s13071-022-05232-8.
Link to cite this reference
Journal title
Issue Date

Files in This Item:

Size: 1.94 MB
Format: Adobe PDF
Show full item record

This item is licensed under a Creative Commons License Creative Commons