Presence of adhesin related genes in enteroaggregative Escherichia coli strains causing diarrhea

Admin Tools
Translated title
Presença de genes relacionados com adesinas em amostras de Escherichia coli enteroagregativa causadoras de diarreia

Responsible researcher
Butantan affiliation
Publication type
Dataset
Language
English
File format
pdf
Updated on
13-Jul-2022
Access rights
Open access
Appears in Collections:
Dataset description
PCR screening for AAF I-IV (.pdf): Multiplex PCR was used to detect the genes encoding the major pilin subunits of AAF/I to IV (aggA, aafA, agg3A and agg4A, respectively), and agg3/4C, corresponding to the usher of AAF/III, IV and V in 110 aatA+ EAEC strains. PCR screening for AAF V (.pdf): Simplex PCR was used to detect the presence of agg5A, encoding the AAF/V major pilin subunit in 110 aatA+ EAEC strains. PCR screening for AFP (.pdf): Simplex PCR was used to detect the presence of afpA1 and afpR, which are part of the AFP-encoding operon in 110 aatA+ EAEC strains. PCR screening for aggR (.pdf): Simplex PCR was used to detect the presence of aggR, encoding the master EAEC virulence regulator in 110 aatA+ EAEC strains. PCR screening for cseA (.pdf): Simplex PCR was used to detect the presence of cseA, encoding the ETEC colonization factor CS22 in 110 aatA+ EAEC strains. List of aatA+ EAEC strains and compiled data (.pdf): All the 110 aatA+ strains were submitted to adherence assays to determine their adherence pattern on HEp-2 cells. They were also submitted to polymerase chain reactions (PCR) analysis to detect the following virulence genes: aggA, aafA, agg3A and agg4A (genes respectively encoding the major pilin subunits of AAF/I to IV); agg3/4C (usher of AAF/III, IV and V); agg5A (encoding the AAF/V major pilin subunit); aggR (encoding the master EAEC virulence regulator); afpA1 and afpR (part of the AFP-encoding operon); and cseA (encoding the ETEC colonization factor CS22).
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4425
Issue Date
2022


Files in This Item:

List of aatA+ EAEC strains and compiled data.pdf
Description: All the 110 aatA+ strains were submitted to adherence assays to determine their adherence pattern on HEp-2 cells. They were also submitted to polymerase chain reactions (PCR) analysis to detect the following virulence genes: aggA, aafA, agg3A and agg4A (genes respectively encoding the major pilin subunits of AAF/I to IV); agg3/4C (usher of AAF/III, IV and V); agg5A (encoding the AAF/V major pilin subunit); aggR (encoding the master EAEC virulence regulator); afpA1 and afpR (part of the AFP-encoding operon); and cseA (encoding the ETEC colonization factor CS22).
Size: 383.29 kB
Format: Adobe PDF
View/Open
PCR screening for AAF V.pdf
Description: Simplex PCR was used to detect the presence of agg5A, encoding the AAF/V major pilin subunit in 110 aatA+ EAEC strains.
Size: 353.22 kB
Format: Adobe PDF
View/Open
PCR screening for AFP.pdf
Description: Simplex PCR was used to detect the presence of afpA1 and afpR, which are part of the AFP-encoding operon in 110 aatA+ EAEC strains.
Size: 381.13 kB
Format: Adobe PDF
View/Open
PCR screening for aggR.pdf
Description: Simplex PCR was used to detect the presence of aggR, encoding the master EAEC virulence regulator in 110 aatA+ EAEC strains.
Size: 584.86 kB
Format: Adobe PDF
View/Open
PCR screening for cseA.pdf
Description: Simplex PCR was used to detect the presence of cseA, encoding the ETEC colonization factor CS22 in 110 aatA+ EAEC strains.
Size: 462.81 kB
Format: Adobe PDF
View/Open
PCR screening for AAF I-IV.pdf
Description: Multiplex PCR was used to detect the genes encoding the major pilin subunits of AAF/I to IV (aggA, aafA, agg3A and agg4A, respectively), and agg3/4C, corresponding to the usher of AAF/III, IV and V in 110 aatA+ EAEC strains.
Size: 590.59 kB
Format: Adobe PDF
View/Open
Show full item record  Duplicate Check

The access to the publications deposited in this repository respects the licenses from journals and publishers.