ZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strains

Publication type
Access rights
Open access
Terms of use
Appears in Collections:
Recent outbreaks of Zika virus (ZIKV) infection have highlighted the need for a better understanding of ZIKV-specific immune responses. The ZIKV envelope glycoprotein (EZIKV) is the most abundant protein on the virus surface and it is the main target of the protective immune response. EZIKV protein contains the central domain (EDI), a dimerization domain containing the fusion peptide (EDII), and a domain that binds to the cell surface receptor (EDIII). In this study, we performed a systematic comparison of the specific immune response induced by different EZIKV recombinant proteins (EZIKV, EDI/IIZIKV or EDIIIZIKV) in two mice strains. Immunization induced high titers of E-specific antibodies which recognized ZIKV-infected cells and neutralized the virus. Furthermore, immunization with EZIKV, EDI/IIZIKV and EDIIIZIKV proteins induced specific IFNγ-producing cells and polyfunctional CD4+ and CD8+ T cells. Finally, we identified 4 peptides present in the envelope protein (E1–20, E51–70, E351–370 and E361–380), capable of inducing a cellular immune response to the H-2Kd and H-2Kb haplotypes. In summary, our work provides a detailed assessment of the immune responses induced after immunization with different regions of the ZIKV envelope protein.
Lunardelli VAS, Apostolico JS, Souza HFS, Coirada FC, Martinho JA, Astray RM, et al. ZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strains. Sci Rep. 2022 Sep; 12: 15733. doi:10.1038/s41598-022-20183-x.
Link to cite this reference
Journal title
Issue Date

Files in This Item:

Existing users please Login
Size: 4.74 MB
Format: Adobe PDF
Embargoed until January 1, 2099    Request a copy
Show full item record

This item is licensed under a Creative Commons License Creative Commons