Purificação de imunoglobulinas de plasma humano empregando Cromatografia de troca iônica

Abstract

Derivatives of human plasma are essential for use in public health in the prevention and treatment of different diseases. Worldwide, the blood products market is leading and competitive. IgG is the blood product of greatest interest and accounts for most of the world market, however IgM has also become a target of interest, due to the efficiency of the pentamer form contributing with more benefits compared to IgG. In this project, the main objective was to separate the fractions containing IgG and IgM immunoglobulins from human plasma using liquid chromatography. In the first stage, the ANX Sepharose FF anion exchange resin was used, in which 98 % of the proteins present in human plasma were collected in the FT, and this fraction was applied to the second anion column in Q Sepharose FF, using a gradient of pH and saline. It was possible to obtain IgG in the FT, albumin in pH 5.5 and IgM in pH 4.5. The 3 fractions obtained require additional purification steps to obtain the final product.