Expressão e purificação da proteína nucleocapsídica recombinante de SARS-CoV-2

Abstract

Known as the Covid-19 Pandemic, caused by the SARS-Cov 2 virus, it became known as one of the great challenges of the 21st century and brought to industry and research the need to create defensive parameters and of rapid effectiveness for the biological study of possible threats to the health of the general population. The Sars-COV-2 Nucleocapsid protein, responsible for stabilizing the nucleocapsid, in addition to being involved in RNA synthesis and translation, and acting as an interferon antagonist and RNA interference suppressor, favoring viral replication, became the target of this study with the objective of creating parameters for its production in the laboratory in the form of recombinant protein. Aiming at high yield and good quality, through its expression in competent bacteria Escherichia coli BL21(DE3) and the improvement in the steps of its creation, counting on sonication of the expressed bacteria, its purification and protein concentration. The present study reached the objectives for the production of this protein for the Laboratório de Imunoquímica of the Instituto Butantan, also being able to guide the production of this and other recombinant proteins that end up becoming of interest for study and research in search of the health of the population.