Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach

Antiangiogenic strategies are promising tools for cancer treatment and several other disorders. In this sense, phospholipases A2 (PLA2s) from snake venom have been described to possess antiangiogenic properties. In this study, we evaluated both in vitro and ex vivo antiangiogenic effects induced by BnSP-7, a Lys49 PLA2 isolated from Bothrops pauloensis snake venom. BnSP-7 was able to inhibit endothelial cell (HUVEC) proliferation, which was indeed confirmed by a modulation of cell cycle progression. Interestingly, BnSP-7 also inhibited the adhesion and migration of HUVECs and blocked in vitro angiogenesis in a VEGF-dependent manner, an important proangiogenic factor. Finally, BnSP-7 was capable of inhibiting sprouting angiogenic process through an ex vivo aortic ring assay. Taken together, these results indicate that BnSP-7 has potent in vitro and ex vivo antiangiogenic effect.
Keywords
PLA2;  Bothrops pauloensis;  Antiangiogenic;  Cancer;  BnSP-7

metadata.dc.contributor.external
metadata.dc.description.sponsorship
Document type
Article
Source
Polloni L, Azevedo FVPV, Teixeira SC, Moura E, Costa TR, Gimenes SNC, et al. Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach. Toxicol. In Vitro. 2021 Jan;in press:105099. doi:10.1016/j.tiv.2021.105099.
Appears in Collections:
Metrics
URL
URI

Files in This Item:
File Description SizeFormat Existing users please Login
doi.org10.1016j.tiv.2021.105099.pdf1.83 MBAdobe PDFEmbargoed until January 1, 2999    Request a copy
Show full item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.