Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach
Polloni, Lorena; Azevedo, Fernanda Van Petten Vasconcelos; Teixeira, Samuel Cota; Moura, Eloá; Costa, Tassia Rafaela; Gimenes, Sarah Natalie Cirilo ; Correia, Lucas Ian Veloso; Freitas, Vitor; Yoneyama, Kelly Aparecida Geraldo; Rodrigues, Renata Santos; Lopes, Daiana Silva; Ávila, Veridiana de Melo Rodrigues
Antiangiogenic strategies are promising tools for cancer treatment and several other disorders. In this sense, phospholipases A2 (PLA2s) from snake venom have been described to possess antiangiogenic properties. In this study, we evaluated both in vitro and ex vivo antiangiogenic effects induced by BnSP-7, a Lys49 PLA2 isolated from Bothrops pauloensis snake venom. BnSP-7 was able to inhibit endothelial cell (HUVEC) proliferation, which was indeed confirmed by a modulation of cell cycle progression. Interestingly, BnSP-7 also inhibited the adhesion and migration of HUVECs and blocked in vitro angiogenesis in a VEGF-dependent manner, an important proangiogenic factor. Finally, BnSP-7 was capable of inhibiting sprouting angiogenic process through an ex vivo aortic ring assay. Taken together, these results indicate that BnSP-7 has potent in vitro and ex vivo antiangiogenic effect.
PLA2; Bothrops pauloensis; Antiangiogenic; Cancer; BnSP-7
Polloni L, Azevedo FVPV, Teixeira SC, Moura E, Costa TR, Gimenes SNC, et al. Antiangiogenic effects of phospholipase A2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach. Toxicol. In Vitro. 2021 Jan;in press:105099. doi:10.1016/j.tiv.2021.105099.
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