Avaliação da transferência passiva de imunoglobulinas específicas do gênero Bothrops de éguas hiperimunizadas para o neonato

Abstract

The placenta epiteliocorial mare, prevents the passage of macromolecules such as IgG's immunoglobulins to the fetus, causing the newborn come to highly susceptible to exogenous microorganisms world, depending exclusively colostrum, rich in mother immunoglobulins, which will give you protection passively during the first hours of life. This work aimed to evaluate the transfer of specific immunoglobulins Bothrops of hipperimmunized mares during the sixth month of pregnancy to the newborn, using the ELISA test (enzyme-linked immuno sorbent) by checking the correlation of specific immunoglobulin concentration maternal serum for the newborn. Five mares and five neonates were used for this purpose. The first blood sample was collected from the mares seven days before they gave birth. The deliveries were followed and samples of newborns followed the following times: T0 before ingestion of colostrum, T1 after 24 hours of colostrum intake, T2 48 hours after ingestion of colostrum and T3 30 days after birth. They were processed by the Hyperimmune Plasma Processing Section- Instituto Butantan. It was observed that three mares obtained the transfer of specific immunoglobulins for newborn, keeping the constant level of immunoglobulins in 12h, 48h and 30 days after birth. A mare did not obtain the necessary value to be considered hyperimmunized, so there was no transfer to the neonate. And another mare obtained a titration considered for hyperimmunization however, its neonate had no specific immunoglobulin value found in the serum at all times.