Genetic and phenotypic characterization of an enteroaggregative Escherichia coli isolated from a urinary tract infection

Biofilm Urine polystyrene (.pdf): absorbance values of biofilm formation in human pooled urine on polystyrene surface. Biofilm Urine glass (.pdf): absorbance values of biofilm formation in human pooled urine on glass surface. Growth curve LB medium (.pdf): growth curve of UPEC-46 and derivative constructions in LB medium – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve DMEM (.pdf): growth curve of UPEC-46 and derivative constructions in DMEM – absorbance values at 600 nm measured every 30 minutes during 6 hours. Growth curve RPMI (.pdf): growth curve of UPEC-46 and derivative constructions in RPMI – absorbance values at 600 nm measured every 30 minutes during 6 hours. Motility (.pdf): halo diameter (cm) of UPEC-46 and derivative constructions on LB agar containing 0.3% agar. Quantitative_adherence HeLa cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HeLa cells – CFU/ml counting. Quantitative_adherence HT-29 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in HT-29 cells – CFU/ml counting. Quantitative_adherence 5637 cells (.pdf): quantitative adherence assays (3 and 6 hours of incubation) of UPEC-46 and derivative constructions in 5637 cells – CFU/ml counting. Steps of afpA mutagenesis (.pdf): steps employed to construct the afpA mutant strain using wild-type UPEC-46 – primers and PCR amplicons analyzed by agarose gel electrophoresis. Steps of afpA mutant complementation (.pdf): steps employed to complement the UPEC-46 mutant in afpA strain – PCR amplicons analyzed by agarose gel electrophoresis. Sanger sequencing of afpA mutant-forward (.txt): forward nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant-reverse (.txt): reverse nucleotide sequence of 4.1 kb PCR amplicon using UPEC-46 afpA mutant as template. Sanger sequencing of afpA mutant complementation-forward (.txt): forward nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Sanger sequencing of afpA mutant complementation- reverse (.txt): reverse nucleotide sequence of 650 bp PCR amplicon using UPEC-46 afpA mutant complemented as template. Prokka UPEC-46 general data (.txt): general data of whole genome sequence of UPEC-46 using Prokka software. Quast UPEC-46 genome quality (.pdf): quality parameters of UPEC-46 genome sequence using Quast software. UPEC-46 virulence genes hits (.xlsx): analysis of the presence of several E. coli virulence genes in the genome of UPEC-46 – identity and coverage. afpA positive strains final binary matrix (.xlsx): binary matrix of presence/absence of several E. coli virulence genes in the genome of UPEC-46, where zero=absence and 1=presence. RAxML_Phylogenetic Tree E. coli reference strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and reference E. coli strains. RAxML_Phylogenetic Tree UPEC, EAEC and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and UPEC and EAEC reference strains. RAxML_Phylogenetic Tree ST10 and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and ST10 E. coli strains. RAxML_Phylogenetic Tree afpA-positive E. coli strains and UPEC-46 (.txt): RAxML file used to generate the phylogram containing UPEC-46 strain and afpA-positive E. coli strains.