Role of the serine protease Sat in the pathogenesis of sepsis caused by Escherichia coli

Translated title
Papel da serinoprotease Sat na patogênese da sepse causada por Escherichia coli

Responsible researcher
Butantan affiliation
Publication type
Dataset
Language
Portuguese;  English
File format
pdf
Access rights
Open access
Appears in Collections:
Dataset description
EC071 25% serum resistance – CFU.mL-1 counting (.pdf): colony forming units counting for bacterial survival after serum resistance assay. EC071 25% serum resistance – graph (.pdf): graphic representation of colony forming units counting for bacterial survival after serum resistance assay. EC071 plasmid analysis (.pdf): plasmid extraction analysis in an agarose gel. Genetic constructions and modifications – agarose gels: PCR, digestion and plasmid extraction products analysis in agarose gels. Growth curve – CFT073 (.pdf): growth curve of CFT073 in LB medium. Growth curve – EC071 and derivative strains (.pdf): growth curve of EC071 and derivative strains in LB medium. Growth curve – MG1655 and derivative strains (.pdf): growth curve of MG1655 and derivative strains in LB medium. Growth curve graphs – CFT073, MG1655 and derivative strains (.pdf): growth curve of CFT073, MG1655 and derivative strains in LB medium graphic representation. Growth curve graphs – EC071 and derivative strains (.pdf): growth curve of EC071 and derivative strains in LB medium graphic representation. Murine sepsis model – survival graph (.pdf): graphic representation of animal survival in a EC071 and derivative strains induced sepsis model. Murine sepsis model – survival statistics (.pdf): statistical analysis of animal survival in a EC071 and derivative strains induced sepsis model. pCF2 insertion in EC071 sat confirmation (.pdf): alignment of sequenced PCR products for pCF2 interruption of the sat gene in EC071 chromosome. pCF4 site-directed mutagenesis confirmation (.pdf): alignment of sequenced PCR products for nucleotide alterations in pCF4 sat gene. Proteolytic assays Immunoblotting (.pdf): detection of cleavage products after Sat and complement targets incubation. Sat or Sat’ production by pCF3 and pCF4 harboring strains (.pdf): detection of Sat ou Sat’ in TCA-treated culture supernatants of pCF3 and pCF4 harboring strains by SDS-PAGE, followed by silver nitrate staining and Immunoblotting. Sat production by EC071 and derivative strains (.pdf): detection of Sat in TCA-treated culture supernatants of EC071 and derivative strains by SDS-PAGE, followed by silver nitrate staining and Immunoblotting. Sat purification – mass spectrometry (.pdf): mass spectrometry analysis report for Sat identification in post-purification fractions. Sat purification – Silver staining and Immunoblotting: Sat detection in post-purification fractions by SDS-PAGE, followed by silver nitrate staining and Immunoblotting.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/3947
FAPESP grants
2017/14821-7
Issue Date
2021


Files in This Item:

EC071 25% serum resistance - graph.pdf
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EC071 plasmid analysis.pdf
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Growth curve - CFT073.pdf
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Proteolytic assays Immunoblotting.pdf
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