Improved molecular diagnosis of enteroaggregative Escherichia coli (EAEC): Triplex-PCR approach targeting aatA, aggR and afpR

Improved molecular diagnosis of enteroaggregative Escherichia coli (EAEC): Triplex-PCR approach targeting aatA, aggR and afpR

Registo de metadados completa

Campo DC	Valor	idioma
dc.contributor	Lab. Bacteriologia	pt_BR
dc.date.accessioned	2025-02-27T14:36:52Z	-
dc.date.available	2025-02-27T14:36:52Z	-
dc.date.issued	2025	pt_BR
dc.identifier.uri	https://repositorio.butantan.gov.br/handle/butantan/5525	-
dc.description.sponsorship	(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo	pt_BR
dc.format	PDF, XLSX	pt_BR
dc.language.iso	English	pt_BR
dc.rights	Open Access	pt_BR
dc.title	Improved molecular diagnosis of enteroaggregative Escherichia coli (EAEC): Triplex-PCR approach targeting aatA, aggR and afpR	pt_BR
dc.title.alternative	Diagnóstico molecular aperfeiçoado de Escherichia coli enteroagregativa (EAEC): abordagem Triplex-PCR para detecção de aatA, aggR e afpR	pt_BR
dc.type	Dataset	pt_BR
dc.contributor.external	(UNIFESP) Universidade Federal de São Paulo	pt_BR
dc.subject.keyword	Enteroaggregative Escherichia coli	pt_BR
dc.subject.keyword	EAEC	pt_BR
dc.subject.keyword	diagnosis	pt_BR
dc.subject.keyword	Triplex-PCR	pt_BR
dc.subject.keyword	diarrhoea	pt_BR
dc.contributor.butantan	Waldir Pereira Elias Junior\|:Pesquisador\|:Docente\|:Programa de Pós-Graduação em Ciências – Biotecnologia e Bioprocessos\|:Lab. Bacteriologia\|:Autor de correspondência	pt_BR
dc.contributor.butantan	Abe, Cecilia Mari\|:Pesquisador\|:Lab. Bacteriologia\|:Autor de correspondência	pt_BR
dc.sponsorship.butantan	Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/04144-0	pt_BR
dc.identifier.bvscc	BR78.1	pt_BR
dc.subject.researchline	Patogenicidade bacteriana	pt_BR
dc.contributor.researcher	Elias, Waldir Pereira	pt_BR
dc.description.dbindexed	Yes	pt_BR
dc.description.file	PCR screening for aggA, aafA, agg3A and agg4A – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of aggA, aafA, agg3A and agg4A, respectively encoding the AAF/I, AAFF/II, AAF/III and AAF/IV major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for agg5A – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of agg5A encoding the AAF/V major pilin subunit in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for afpA1 – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of afpR, encoding AFP major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for cseA – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of cseA, encoding CS22 major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). Triplex-PCR screening EAEC detection – agarose gels (EAEC strains from Gomes et al) (.pdf): Triplex-PCR to detect the presence of aatA, aggR and afpR in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). Triplex-PCR screening for EAEC detection – agarose gels (EAEC strains from Bueris et al) (.pdf): Triplex-PCR to detect the presence of aatA, aggR and afpR in 110 clinical EAEC strains from Bueris et al. (DOI: 10.1590/s0074-02762007005000116). aatA, aggR and afpR sequences obtained from databases (.xlsx): 508 complete nucleotide sequences (251 aatA, 224 aggR, and 33 afpR) downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov/) in the FASTA format. Three different databases (one for each targeted gene) were constructed by merging all the corresponding sequences in a single text file.	pt_BR
dc.sponsorship.fapesp	2018/04144-0	pt_BR
item.languageiso639-1	English	-
item.grantfulltext	open	-
item.fulltext	Com Texto completo	-
item.openairetype	Dataset	-
Aparece nas Coleções:	Dados de Pesquisa (Dataset)

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PCR screening for aggA, aafA, agg3A and agg4A – agarose gels (EAEC strains from Gomes et al).pdf
Descrição: PCR to detect the presence of aggA, aafA, agg3A and agg4A, respectively encoding the AAF/I, AAFF/II, AAF/III and AAF/IV major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998).
Tamanho: 1.33 MB
Formato: Adobe PDF
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PCR screening for agg5A – agarose gels (EAEC strains from Gomes et al).pdf
Descrição: PCR to detect the presence of agg5A encoding the AAF/V major pilin subunit in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998).
Tamanho: 1.37 MB
Formato: Adobe PDF
Ver/Aberto

PCR screening for afpA1 – agarose gels (EAEC strains from Gomes et al).pdf
Descrição: PCR to detect the presence of afpR, encoding AFP major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998).
Tamanho: 892.41 kB
Formato: Adobe PDF
Ver/Aberto

PCR screening for cseA – agarose gels (EAEC strains from Gomes et al).pdf
Descrição: PCR to detect the presence of cseA, encoding CS22 major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998).
Tamanho: 274.22 kB
Formato: Adobe PDF
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Triplex-PCR screening for EAEC detection – agarose gels (EAEC strains from Gomes et al).pdf
Descrição: Triplex-PCR to detect the presence of aatA, aggR and afpR in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998).
Tamanho: 274.22 kB
Formato: Adobe PDF
Ver/Aberto

Triplex-PCR screening for EAEC detection – agarose gels (EAEC strains from Bueris et al).pdf
Descrição: Triplex-PCR to detect the presence of aatA, aggR and afpR in 110 clinical EAEC strains from Bueris et al. (DOI: 10.1590/s0074-02762007005000116).
Tamanho: 492.75 kB
Formato: Adobe PDF
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aggR-aatA-afpR sequences obtained from databases.xlsx
Descrição: Analyses of multiple Escherichia coli genomes available in the NCBI database (https://www.ncbi.nlm.nih.gov/), focusing on the identification of the aatA, aggR, and afpR genes. As a result, 508 complete nucleotide sequences were selected (251 aatA, 224 aggR, and 33 afpR). Three distinct databases (one for each target gene) were constructed using Sublime Text 4 software (https://www.sublimetext.com/).
Tamanho: 41.79 kB
Formato: Microsoft Excel XML
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