Potêncial antiproliferativo de monofosfoesteres lipídico em células de Glioblastoma humano

Abstract

Glioblastoma multiforme (GBM) is the most common type of malignant primary astrocytoma. It accounts for over 60% of all brain tumors in adults. Despite the variety of modern therapies against GBM, it is still a deadly disease with extremely poor prognosis. Patients usually have an average survival of approximately 14 to 15 months from diagnosis. The aim of this study was to evaluate the cytotoxic and antiproliferative effects of three monophosphoesters (MFE) on U-138 human glioblastoma multiforme cells. The determination of cytotoxic activity of the compounds was evaluated by the MTT colorimetric method. Treatment with MFE-1 presented cytotoxicity to tumor cell U-138, with IC50 values 18.16 and 11.6 mM in the 24 and 48h period, generating morphological changes of toxicity. For the treatment with MFE-2 the IC50% value was 30.22 and 25mM in the 24 and 48h periods, with significant changes in morphology and reduction of confluence. Compound MFE-3 was not effective for in vitro treatment of U-138 tumor cells, with IC50% values 76.74 and 67.8 mM. When comparing the compounds with respect to their in vitro effectiveness, their antiproliferative and cytotoxic capacity for the U-138 glioblastoma multiforme cell, monophosphoester 1 had a lower IC50% in both treatment periods, 24 and 48h, compared to the other compounds. Monophosphoester 3 did not present a significant result, obtaining an IC50% 4 times higher than monophosphoester 1 in the 24h and approximately 6 times higher when comparing the value obtained for the 48h treatment.