Improved molecular diagnosis of enteroaggregative Escherichia coli (EAEC): Triplex-PCR approach targeting aatA, aggR and afpR

PCR screening for aggA, aafA, agg3A and agg4A – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of aggA, aafA, agg3A and agg4A, respectively encoding the AAF/I, AAFF/II, AAF/III and AAF/IV major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for agg5A – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of agg5A encoding the AAF/V major pilin subunit in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for afpA1 – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of afpR, encoding AFP major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). PCR screening for cseA – agarose gels (EAEC strains from Gomes et al) (.pdf): PCR to detect the presence of cseA, encoding CS22 major pilin subunits in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). Triplex-PCR screening EAEC detection – agarose gels (EAEC strains from Gomes et al) (.pdf): Triplex-PCR to detect the presence of aatA, aggR and afpR in 53 clinical EAEC strains from Gomes et al. (DOI: 10.1128/JCM.36.12.3609-3613.1998). Triplex-PCR screening for EAEC detection – agarose gels (EAEC strains from Bueris et al) (.pdf): Triplex-PCR to detect the presence of aatA, aggR and afpR in 110 clinical EAEC strains from Bueris et al. (DOI: 10.1590/s0074-02762007005000116). aatA, aggR and afpR sequences obtained from databases (.xlsx): 508 complete nucleotide sequences (251 aatA, 224 aggR, and 33 afpR) downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov/) in the FASTA format. Three different databases (one for each targeted gene) were constructed by merging all the corresponding sequences in a single text file.