Role of AFP in adherence and colonization of intestinal and urinary tracts

Quantitative adherence and invasion of UPEC-46 (.pdf): quantitative adherence and invasion assays (3 hours of incubation, without D-mannose) of UPEC-46 using different eukaryotic cell lineages. Interaction and invasion ability of UPEC-46 and controls in different cells (.pdf): total interaction, internalized bacteria and invasion of UPEC-46 and control strains (3 hours of incubation, without D-mannose) using different eukaryotic cell lineages. Effect of D-mannose in adherence of UPEC-46 (.pdf): effect of D-mannose on T1P-mediated adherence of UPEC-46 strain in different eukaryotic cell lineages. Quantitative adherence of UPEC-46 and derivate strains in different cells (.pdf): quantitative adherence assays (3 hours of incubation, without D-mannose) of UPEC-46 and derivate strains using different eukaryotic cell lineages. Bladders and kidneys colonization UPEC-46 (.pdf): Bladders and kidneys colonization mediated by UPEC-46 strain in UTI mouse model (6, 12, 24, or 72 h). Urinary colonization UPEC-46 and derivate strains (.pdf): Bladders and kidneys colonization mediated by UPEC-46 and derivate strains in UTI mouse model (72 h). Intestinal colonization UPEC-46 and derivate strains (.pdf): Mice were orogastrically inoculated with 103 CFU of UPEC-46 and derivate strains. Fresh fecal samples were collected from each mouse for up to 14 d post-inoculation for bacteria counts. Area under curve UPEC-46 and derivate strains (.pdf): cumulative bacterial enumeration for 14 d post infection [CFU/g feces]. Growth curve of UPEC-46 and double mutant (.pdf): growth curve of UPEC-46 and double mutant in different culture mediums. Motility of UPEC-46 and double mutant (.pdf): halo diameter (cm) of UPEC-46 and double mutant on LB agar containing 0.3% agar. Steps of fimH mutagenesis (.pdf): steps employed to construct the fimH mutant strain using UPEC-46